Membrane Proteome Array™

De-risk and deorphan antibody programs by choosing the right lead molecule

What does my antibody bind?

Off-target drug binding can cause mild to serious side effects or even death. We developed the Membrane Proteome Array as the largest array of membrane proteins yet assembled for antibody specificity profiling and deorphaning.

Why is the Membrane Proteome Array so successful?

Membrane Proteome Array

  • Largest library for cell-based profiling of full-length membrane proteins - includes >95% of the membrane proteome
  • Flow cytometry for high-sensitivity detection in unfixed human cells
  • Cell-based platform ensures native protein conformations and post-translational modifications

“The team at IM is very scientifically engaged and responsive, and have an excellent turnaround time. The MPA allowed us to move forward with our targeted molecules with confidence of their specificity.”

- SVP RESEARCH AND DEVELOPMENT, CLINICAL STAGE BIOTECH

What makes your MAb profiling approach unique?

We screen the largest human membrane protein library ever assembled using flow cytometry for high-sensitivity detection, resulting in the highest success rates.
 Membrane Proteome ArrayCell MicroarrayProtein Microarray
Size (unique proteins)5,3003,5003,800
Binding conditions unfixed cellsfixed cellscell lysate
Detection methodflow cytometryimmunofluorescenceimmunofluorescence
Hit validationantibody dilution curveadditional service Western blot
Speed4 weeks8 weeks3 weeks

How can the Membrane Proteome Array help me?

Specificity Profiling

We screen your antibody to determine its membrane proteome binding profile, identifying target and off-target interactions

Learn more about how the MPA can de-risk your antibody discovery project

Antibody Deorphaning

We screen your antibody of unknown specificity to identify which protein(s) on the MPA the antibody binds

Learn more about how the MPA can deorphan your antibodies

Phenotypic Target Discovery

We partner with you to discover new therapeutic targets using high-throughput, cell-based functional assays on our MPA library

Learn more about how we use the MPA to discover novel therapeutic targets

What to Expect:

    1. You send us 500µg of your antibody
    2. We determine appropriate detection conditions
    3. We screen your antibody on the Membrane Proteome Array
    4. We validate any identified target protein(s) in a titrated binding experiment
    5. We send you a final report within 4 weeks

“We have been very pleased with the data that we obtained from the Membrane Proteome Array platform. This showed that our antibodies recognize only their select target, and not the over 5,000 other membrane proteins in their panel.”

-MARIA GONZALEZ-PAJUELO, CSO, FAIRJOURNEY BIOLOGICS

Frequently Asked Questions

1How does a Membrane Proteome Array project work?

The Membrane Proteome Array makes use of the largest membrane protein library ever assembled using flow cytometry for high sensitivity detection, resulting in the broadest screens and the highest success rates. A typical project consists of the following:

  • You send us 500µg of your antibody.
  • We optimize conditions for detecting binding of your antibody in our high-throughput system.
  • We screen your antibody on the Membrane Proteome Array using optimized conditions.
  • We confirm all hits in a second flow cytometry experiment.
  • You receive a detailed report containing a membrane protein binding profile and the identity of validated targets.
2What is the turnaround time for a Membrane Proteome Array project?
A typical MPA project takes four weeks from receipt of antibody.
3What are the deliverables that I will receive upon completion of a project?
You receive a detailed report containing a membrane protein binding profile and a second dose-response validation screen on any identified targets.
4What binding conditions do you use for screening the Membrane Proteome Array?
The MPA is very flexible with regard to screening conditions. We typically conduct screens under native conditions, but can use permeabilizing or even serum free conditions if required. We will discuss the best conditions for your antibody with you at the start of a project.
5How do you validate your hits?
Hits are validated by testing serial dilutions of your antibody for binding to cells over-expressing the identified target(s) in a second flow cytometry experiment.

Ready to Get Started?

We have worked with more than 100 top biotech and pharmaceutical companies to help them succeed. Let's talk about how we can help you.
Or call (215) 966-6061