Secreted Proteome Library
Antibody specificity testing against soluble proteins, enabling comprehensive analysis for safety and pharmacokinetics
The highest quality antibody specificity testing service in the field — Now with a secreted protein library
For the most comprehensive picture of your molecule’s off-target specificity, you can expand your Membrane Proteome Array™ (MPA) specificity testing project to include screening on our Secreted Proteome Library (SPL). With 1,200 high-confidence secreted human proteins, this add-on service can provide deeper insights into your molecule’s interactions with the human body—including off-target binding that may affect its safety and its pharmacokinetics.
Secreted Proteome Array Features
We designed the Secreted Proteome Library platform from the ground up and optimized it for secreted proteins — and it is held to the same quality standards you’ve come to expect from our Membrane Proteome Array platform. The library includes growth factors, interleukins, chemokines, interferons, complement factors, proteases, and more.
QUANTITATIVE — Highly sensitive ELISA assay provides quantitative measurements for statistical analysis
COMPATIBLE— Works with all biotherapeutic modalities, including monoclonal antibodies, scFv, VHH, bispecifics, peptides, ADCs, and CAR-T cell therapies
BIOLOGICALLY RELEVANT — Human proteins expressed in human cells to ensure native folding and post-translational modifications
EFFICIENT — Provides additional data for up to 8 antibodies without extending your MPA project timeline
Service Details
You can add SPL screening to any MPA project, for specificity data on a combined total of 7,000+ proteins. The project workflow for SPL and MPA screening follow the same general steps. After assay setup and optimization, we perform ELISA-based screening on the Secreted Proteome Library. Every hit from your screen goes through a validation step, using a dose-response titration experiment, to yield high-confidence binding results. As with the MPA, screening on the SPL reveals the identity of any off-targets that may interact with your molecule.
Project Deliverables
You’ll receive your SPL screening data as a stand-alone report with publication-quality data and figures, including:
- Assay setup data
- Library screen data: binding data for your molecule against the full 1,200-protein SPL, highlighting any validated off-targets
- Hit validation data for validated targets and off-targets
Platform & Assay Design
The MPA’s standard library and screening platform are optimized for the particular challenges of membrane proteins. Because soluble proteins require different conditions for optimization, the SPL was designed from the ground up exclusively for secreted proteins.
- Proteins are produced in human embryonic kidney (HEK) cells
- Cell-free, ELISA format provides the optimal blend of sensitivity, reliability, and native protein biology
- Development and validation of the SPL assay followed the rigorous FDA and EMA guidance for Process Validation and complies with ISO 9001 section 8.3, Design and Development of Products and Services
Protein families included in the Secreted Proteome Library |
|---|
Growth Factors |
Interleukins |
Chemokines |
Interferons |
Complement Factors |
Proteases |
…and much more |
Our commitment to data quality informs everything we do at Integral Molecular, and the SPL is held to the same ISO9001 standards as the MPA platform. For additional details, please contact us.
Frequently Asked Questions
Development and validation of the Secreted Proteome Library and assay followed the rigorous FDA and EMA guidance for Process Validation and complies with ISO 9001 section 8.3, Design and Development of Products and Services. The assay underwent rigorous reproducibility and repeatability experiments on several diverse test molecules and passed validation metrics outlined in the product development Qualification Plan.
We ask for about 1.0 mg of your molecule. That’s enough material for screening on both the Membrane Proteome Array and Secreted Proteome Library.
Because screening on the Secreted Proteome library (SPL) and Membrane Proteome Array (MPA) are performed in parallel, adding SPL screening does not affect the timeline of your MPA project. Following receipt of your antibodies, we deliver results within 8 weeks for standard MPA projects and within 4 weeks for MPA+IND projects.
The SPL contains 1,200 biologically important proteins found in blood plasma. It includes growth factors, interleukins, chemokines, interferons, complement factors, proteases, and more. If there’s a particular protein or group of proteins you’re interested in, contact us and we can let you know if it’s included.