Lipoparticle™ Technology

High-concentration membrane proteins on virus-like particles for antibody discovery

Why do you need Lipoparticles for antibody immunization and screening?

Lipoparticles concentrate conformationally intact membrane proteins directly on the cell surface, enabling these complex proteins to be manipulated as soluble, high-concentration proteins for antibody immunization and screening

Why are Lipoparticles so effective for antibody discovery?

  • Lipoparticles concentrate proteins at 10-100× (~50-200 pmol/mg) the concentration of cells or membrane preps, resulting in robust immune responses and more successful antibody screens
  • Lipoparticles display properly folded membrane proteins in their native cellular membrane, resulting in the ability to elicit and screen for conformational, functional antibodies
  • Lipoparticles are ~150 nM, the size of most viruses, and so are optimal targets for dendritic cells in vivo and surface attachment for phage display

Lipoparticle applications include:

  • Immunization
  • Phage/yeast display
  • Antibody screening by ELISA
  • Kinetic analysis by biosensor
  • Radioligand and fluorescent binding assays

Lipoparticle applications include:

  • Immunization
  • Phage/yeast display
  • Antibody screening by ELISA
  • Kinetic analysis by biosensor
  • Radioligand and fluorescent binding assays

"For my custom Lipoparticle project I really appreciated that Integral Molecular was not satisfied with average quality." The team did all they could do to meet our expectations and they succeeded."

-Pieter Fokko van Loo, Pre-clinical Program Director, Merus

How can I buy Lipoparticles?

Custom Lipoparticles

Lipoparticles are typically customized to incorporate customers’ specified membrane proteins. Each Lipoparticle batch is assessed by using up to 16 quality-control metrics to ensure homogeneity, purity, and target protein integrity. Hundreds of membrane proteins have been successfully incorporated into Lipoparticles, which can be modified with biotin or fluorescence for detection.

ReadyReceptor Lipoparticles

Prevalidated Lipoparticles contain optimized, highly expressed membrane proteins and are available for rapid delivery. ReadyReceptor Lipoparticles are produced and validated by using the same stringent quality metrics as are used for our Custom Lipoparticle production.

Protein Class

1GPCR
ReceptorDescriptionCatalog No.
A2ARAlpha AdrenergicRR-0910
B2ARBeta AndrenergicRR-0574
CCR1ChemokineRR-0968
CCR2bChemokineRR-0125
hCCR5ChemokineRR-0190
mCCR5ChemokineRR-0835
rhCCR5ChemokineRR-0895
CCR6ChemokineRR-0822
CCR10ChemokineRR-0193
CXCR2ChemokineRR-0943
CXCR3ChemokineRR-0132
CXCR4ChemokineRR-0830
CXCR7ChemokineRR-0656
mCXCR4ChemokineRR-2194
GLP-1RGlucagon-like peptide-1 ReceptorRR-1090
GCGRGlucagon ReceptorRR-0999
GAL1 ReceptorNeuropeptide ReceptorRR-1067
5-HT2CSerotonin ReceptorRR-1498
FZD4Wnt PathwayRR-1302
2Ion Channel
ReceptorDescriptionCatalog No.
HV1Voltage Gated H+RR-0678
TRPC4Nonspecific Cation ChannelRR-1054
TRPM4Nonspecific Cation ChannelRR-1333
K2P1.1Potassium ChannelRR-1225
K2P4.1Potassium ChannelRR-1331
P2X2ATP PurinoceptorRR-1181
3Transporter
ReceptorDescriptionCatalog No.
GLUT1Glucose Transporter RR-0777
4RTK
ReceptorDescriptionCatalog No.
ALK2Activin ReceptorRR-0850
51-TM
ReceptorDescriptionCatalog No.
ACVR1Activin Receptor, Type 1RR-0850
CD19B-Cell ActivationRR-0553
CD27B-Cell ActivationRR-0655
DC-SIGNLectinRR-0057
DC-SIGNRLectinRR-0056
LRP6Wnt PathwayRR-0853
hTL1ATNF LigandRR-0819
mTL1ATNF LigandRR-0817
DR5TNF-family ReceptorRR-0818
ST2LIL-1 Receptor FamilyRR-0653
64-TM
ReceptorDescriptionCatalog No.
CD81Hepatitis C Attachment RR-0341
CD20 B-Cell Activation RR-1179
Claudin-1 Tight Junction RR-0825
Claudin-3 Tight Junction RR-0733
Claudin-4 Tight Junction RR-0686
7Viral Protein
ReceptorDescriptionCatalog No.
RSV-FRespiratory Syncytial Virus RR-0891
Influenza M2 A/Udorn/307/1972 Ion Channel RR-0929
Influenza M2 A/HK/156/1997Ion Channel RR-0179
Chikungunya VirusViral Envelope RR-0945
Ross River Virus Viral Envelope RR-1136

Sample Kits

Lipoparticle sample kits containing CXCR4, CCR5 or CD20 are available for overnight delivery to enable application optimization and small-scale testing.

Sample Kit Class Cat. No Size
CXCR4GPCRLEV-101, LEV-101B
400 U
CCR5 GPCRLEV-102, LEV-102B400 U
CD20GPCRLEV-103, LEV-103B400 U

Lipoparticles can be modified with (B) Biotin or (F) Fluorescence.

80+ Lipoparticle Publications & Patents

"Integral Molecular is the only source available for full length CXCR4 protein for ligand binding assays. Both human and mouse CXCR4 Lipoparticles are available, which was essential for the preclinical project that I was working on."

"The customer service is great and there are a wide variety of membrane proteins."

- ANGELA STAUFFER, SCIENTIST, PFIZER

Frequently Asked Questions

1What are Lipoparticles?
Lipoparticles are noninfectious virus-like particles (VLPs) containing high concentrations of specific membrane proteins in their native conformation.
2What is a Lipoparticle Unit?
A Lipoparticle Unit correlates with the number of Lipoparticles in solution. One Unit is enough to obtain a strong ELISA response from 1 well of a 96-well plate. Lipoparticles are normalized by Dynamic Light Scattering (DLS). The DLS intensity is proportional to the number of Lipoparticles in solution.
3Are target membrane proteins on Lipoparticles conformationally intact?
Yes. Target membrane proteins are conformationally intact because they are incorporated onto the Lipoparticle surface directly from the cell surface and are never extracted, detergent solubilized, or refolded. Lipoparticles are usually produced from human cell lines, so receptors also contain native post-translational modifications. Conformational integrity is confirmed by binding to conformation-dependent antibodies or ligands, depending on the receptor and available reagents.
4Can complexes of multiple membrane proteins (e.g., two or more) be incorporated?
Yes, two or more membrane proteins can be incorporated simultaneously while maintaining their association as multiprotein complexes.
5How stable are Lipoparticles?
Lipoparticle stability is comparable to the stability of other purified proteins. Lipoparticles are stable for at least 2 years when refrigerated or frozen (-80°C). Like most lipid structures, Lipoparticles will be disrupted by detergents. Lipoparticle suspensions will appear as an opaque solution and should be gently mixed before use. Excessive vortexing, dilution, and filtration of Lipoparticles should be avoided to minimize sample loss.
6Can Lipoparticles be labeled or modified?
Lipoparticles are nonliving biochemical reagents and can readily be modified during the production and purification process. Lipoparticles are available from Integral Molecular as biotinylated or fluorescently labeled molecules.
7Do Lipoparticles contain nonspecific membrane proteins?
Lipoparticles bud from the cell surface, so they contain specific membrane proteins of interest as well as additional membrane proteins from the cells that are used for production. The effects of these nonspecific membrane proteins are minimized by the overexpression of the membrane protein of interest, the use of alternative cell types where necessary, and the use of Null Lipoparticles that do not contain the membrane protein of interest as negative controls.

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