Why do I need Lipoparticles for antibody immunization and screening?
Why are Lipoparticles so effective for antibody discovery?
- Lipoparticles concentrate proteins at 10-100× (~50-200 pmol/mg) that of cells or membrane preps, resulting in robust immune responses and more successful antibody screens
- Lipoparticles display correctly folded membrane proteins in their native cellular membrane, resulting in the ability to elicit and screen for conformational, functional antibodies
- Lipoparticles are ~150 nM, the size of most viruses, and so are optimal targets for dendritic cells in vivo and surface attachment for phage display
Lipoparticle applications include:
- Phage/yeast display
- Antibody screening by ELISA
- Kinetic analysis by biosensor
- Radioligand and fluorescent binding assays
"For my custom Lipoparticle project I really appreciated that Integral Molecular was not satisfied with average quality." The team did all they could do to meet our expectations and they succeeded."-Pieter Fokko van Loo, Pre-clinical Program Director, Merus
How can I buy Lipoparticles?
Lipoparticles are typically customized to incorporate customers’ specified membrane proteins. Each Lipoparticle batch is assessed using up to 16 quality-control metrics to ensure homogeneity, purity, and target protein integrity. Hundreds of membrane proteins have been successfully incorporated into Lipoparticles, which can be modified with biotin or fluorescence for detection.
Prevalidated Lipoparticles contain optimized, highly expressed membrane proteins and are available for rapid delivery. ReadyReceptor Lipoparticles are produced and validated using the same stringent quality metrics as are used for our Custom Lipoparticle production.
|GLP-1R||Glucagon-like peptide-1 Receptor||RR-1090|
|GAL1 Receptor||Neuropeptide Receptor||RR-1067|
|HV1||Voltage Gated H+||RR-0678|
|TRPC4||Nonspecific Cation Channel||RR-1054|
|TRPM4||Nonspecific Cation Channel||RR-1333|
|ACVR1||Activin Receptor, Type 1||RR-0850|
|ST2L||IL-1 Receptor Family||RR-0653|
|CD81||Hepatitis C Attachment||RR-0341
|RSV-F||Respiratory Syncytial Virus||RR-0891
|Influenza M2 A/Udorn/307/1972||Ion Channel||RR-0929
|Influenza M2 A/HK/156/1997||Ion Channel||RR-0179
|Chikungunya Virus||Viral Envelope||RR-0945
|Ross River Virus||Viral Envelope||RR-1136
Lipoparticle sample kits containing CXCR4, CCR5 or CD20 are available for overnight delivery to enable application optimization and small-scale testing.
|Sample Kit||Class||Cat. No||Size|
|CXCR4||GPCR||LEV-101, LEV-101B||400 U
|CCR5||GPCR||LEV-102, LEV-102B||400 U
|CD20||GPCR||LEV-103, LEV-103B||400 U|
Lipoparticles can be modified with (B) Biotin or (F) Fluorescence.
80+ Lipoparticle Publications & Patents
Featured Case Studies
"Integral Molecular is the only source available for full length CXCR4 protein for ligand binding assays. Both human and mouse CXCR4 Lipoparticles are available, which was essential for the preclinical project that I was working on."
"The customer service is great and there are a wide variety of membrane proteins."- ANGELA STAUFFER, SCIENTIST, PFIZER