Lipoparticle Technology

High concentration membrane proteins on virus-like particles, for drug and antibody discovery.

Why do I need Lipoparticles for antibody immunization and screening?

Lipoparticles are virus-like particles (VLPs) containing high concentrations of specific membrane proteins in their native conformation. Lipoparticles capture conformationally-intact membrane proteins directly from the cell surface, enabling these complex proteins to be manipulated as soluble proteins for antibody immunization and screening.

The Lipoparticle

Why are Lipoparticles such effective antigens?

  • Lipoparticles display correctly folded membrane proteins in their native cellular membrane. This results in the ability to elicit and screen for conformational antibodies.
  • Lipoparticles contain 10-100 fold more concentrated membrane proteins (~50-200 pmol/mg) compared to cells, resulting in robust immune responses and better success in antibody screening.

Lipoparticle applications include:

  • Immunization
  • Phage/yeast display
  • Antibody screening by ELISA
  • Kinetic analysis by biosensor
  • Radioligand and fluorescent binding assays

"For my custom Lipoparticle project I really appreciated that Integral Molecular was not satisfied with average quality."

"The team did all they could do to meet our expectations and they succeeded."

-Pieter Fokko van Loo, Pre-clinical Program Director, Merus

How can I buy Lipoparticles?

Custom Lipoparticles

Lipoparticles are customized to incorporate any membrane protein of choice. Each batch of Lipoparticles is assessed using quality control metrics to ensure homogeneity, purity, and target protein integrity. Hundreds of membrane protein targets have been successfully incorporated into Lipoparticles. Lipoparticles can be modified with biotin or fluorescence for specific applications.

ReadyReceptor Lipoparticles

Pre-validated Lipoparticles contain optimized, highly-expressed membrane proteins and are available for rapid delivery. ReadyReceptor Lipoparticles are produced and validated using all of the stringent quality metrics used for Custom Lipoparticle production.

Protein Class

1GPCR
ReceptorDescriptionCatalog No.
A2ARAlpha AdrenergicRR-0910
B2ARBeta AndrenergicRR-0574
C5ARComplementRR-0362
CCR1ChemokineRR-0968
CCR2bChemokineRR-0125
hCCR5ChemokineRR-0190
mCCR5ChemokineRR-0835
rhCCR5ChemokineRR-0895
CCR6ChemokineRR-0822
CCR10ChemokineRR-0193
CXCR2ChemokineRR-0943
CXCR3ChemokineRR-0132
CXCR4ChemokineRR-0830
CXCR7ChemokineRR-0656
mCXCR4ChemokineRR-2194
GLP-1RGlucagon-like peptide-1 ReceptorRR-1090
GCGRGlucagon ReceptorRR-0999
GAL1 ReceptorNeuropeptide ReceptorRR-1067
5-HT2CSerotonin ReceptorRR-1498
FZD4Wnt PathwayRR-1302
2Ion Channel
ReceptorDescriptionCatalog No.
HV1Voltage Gated H+RR-0678
TRPC4Nonspecific Cation ChannelRR-1054
TRPM4Nonspecific Cation ChannelRR-1333
K2P1.1Potassium ChannelRR-1225
K2P4.1Potassium ChannelRR-1331
P2X2ATP PurinoceptorRR-1181
3RTK
ReceptorDescriptionCatalog No.
ALK2Activin ReceptorRR-0850
41-TM
ReceptorDescriptionCatalog No.
ACVR1Activin Receptor, Type 1RR-0850
CD19B-Cell ActivationRR-0553
CD27B-Cell ActivationRR-0655
DC-SIGNLectinRR-0057
DC-SIGNRLectinRR-0056
LRP6Wnt PathwayRR-0853
hTL1ATNF LigandRR-0819
mTL1ATNF LigandRR-0817
DR5TNF-family ReceptorRR-0818
ST2LIL-1 Receptor FamilyRR-0653
54-TM
ReceptorDescriptionCatalog No.
CD81Hepatitis C Attachment RR-0341
CD20 B-Cell Activation RR-1179
Claudin-1 Tight Junction RR-0825
Claudin-3 Tight Junction RR-0733
Claudin-4 Tight Junction RR-0686
6Viral Protein
ReceptorDescriptionCatalog No.
RSV-FRespiratory Syncytial Virus RR-0891
Influenza M2 A/Udorn/307/1972 Ion Channel RR-0929
Influenza M2 A/HK/156/1997Ion Channel RR-0179
Chikungunya VirusViral Envelope RR-0945
Ross River Virus Viral Envelope RR-1136
7Transporter
ReceptorDescriptionCatalog No.
GLUT1Glucose Transporter RR-0777

Sample Kits

Lipoparticle sample kits containing CXCR4, CCR5 or CD20 are available for overnight delivery to enable application optimization and small-scale testing.

Sample Kit Class Cat. No Size
CXCR4GPCRLEV-101, LEV-101B
400 U
CCR5 GPCRLEV-102, LEV-102B400 U
CD20GPCRLEV-103, LEV-103B400 U

Lipoparticles can be modified with (B) Biotin or (F) Fluorescence.

Lipoparticle Publications

"Integral Molecular is the only source available for full length CXCR4 protein for ligand binding assays. Both human and mouse CXCR4 Lipoparticles are available, which was essential for the preclinical project that I was working on."

"The customer service is great and there are a wide variety of membrane proteins."

- ANGELA STAUFFER, SCIENTIST, PFIZER

Frequently Asked Questions

1What are Lipoparticles?
Lipoparticles are non-infectious virus-like particles (VLPs) containing high concentrations of specific membrane proteins in their native conformation.
2What is the concentration of the target membrane protein that is incorporated onto Lipoparticles?
Lipoparticles typically contain 50-200 pmol/mg of a specific target membrane protein, which corresponds to approximately 50-200 specific receptors per Lipoparticle. Membrane protein incorporation is directly related to the amount of the membrane protein expressed at the cell surface.
3Are target membrane proteins on Lipoparticles conformationally-intact?
Yes, target membrane proteins are conformationally-intact because they are incorporated onto the surface of Lipoparticles directly from the cell surface and are never extracted or refolded. Lipoparticles are usually produced from human cell lines so receptors also contain native post-translational modifications. Conformational integrity is demonstrated using conformation-dependent antibodies or ligand binding, depending on the receptor and available reagents.
4Can complexes of multiple membrane proteins (e.g. 2 or more) be incorporated?
Yes, two or more membrane proteins can be incorporated simultaneously while maintaining their association as multi-protein complexes.
5How stable are Lipoparticles?
Lipoparticle stability is comparable to other purified proteins. Lipoparticles are stable for at least 6 months when refrigerated or frozen. Like most lipid structures, Lipoparticles will be disrupted by detergents, but are stable in the presence of small amounts (<5%) of common additives (e.g. DMSO, ethanol). Lipoparticle suspensions will appear as an opaque solution and should be gently mixed prior to use. Excessive vortexing, dilution, and filtration of Lipoparticles should be avoided to minimize sample loss.
6Can Lipoparticles be labeled or modified?
Lipoparticles are non-living biochemical reagents, so can readily be modified during the production and purification process. Lipoparticles are available from Integral Molecular biotinylated or fluorescently labeled.
7Do Lipoparticles contain non-specific membrane proteins?
Lipoparticles bud from the cell surface, so they contain specific membrane proteins of interest as well as additional membrane proteins from the cells used for production. The effects of non-specific membrane proteins are minimized by over-expression of the membrane protein of interest, the use of alternative cell types where necessary, and the use of Null Lipoparticles that do not contain the membrane protein of interest as negative controls.

Ready to Get Started?

We have worked with more than 100 top biotech and pharmaceutical companies to help them succeed. Let's talk about how we can help you.
Or call (215) 966-6061