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LIPOPARTICLE™ TECHNOLOGY
High-concentration membrane proteins on virus-like particles for antibody discovery
GPCRs, ion channels, transporters, and I-O targets
Proteins at 10-100× cell concentration
2000+
Lipoparticle projects completed in 18 years
Why do you need Lipoparticles for antibody immunization and screening?
Lipoparticles concentrate conformationally intact membrane proteins directly on the cell surface, enabling these complex proteins to be manipulated as soluble, high-concentration proteins for antibody immunization and screening
Why are Lipoparticles so effective for antibody discovery?
- Lipoparticles concentrate proteins at 10-100× (~50-200 pmol/mg) the concentration of cells or membrane preps, resulting in robust immune responses and more successful antibody screens
- Lipoparticles display properly folded membrane proteins in their native cellular membrane, resulting in the ability to elicit and screen for conformational, functional antibodies
- Lipoparticles are ~150 nm, the size of most viruses, and so are optimal targets for dendritic cells in vivo and surface attachment for phage display
Lipoparticle applications include:
- Immunization
- Phage/yeast display
- Antibody screening by ELISA
- Kinetic analysis by biosensor
- Radioligand and fluorescent binding assays
"For my custom Lipoparticle project I really appreciated that Integral Molecular was not satisfied with average quality. The team did all they could do to meet our expectations and they succeeded."
How can I buy Lipoparticles?
Custom Lipoparticles
Lipoparticles are typically customized to incorporate customers’ specified membrane proteins. Each Lipoparticle batch is assessed by using up to 16 quality-control metrics to ensure homogeneity, purity, and target protein integrity. Hundreds of membrane proteins have been successfully incorporated into Lipoparticles, which can be modified with biotin or fluorescence for detection.
Ready Receptor Lipoparticles
Prevalidated Lipoparticles contain optimized, highly expressed membrane proteins and are available for rapid delivery. ReadyReceptor Lipoparticles are produced and validated by using the same stringent quality metrics as are used for our Custom Lipoparticle production.
Protein Class
Sample Kits
Lipoparticle sample kits containing CXCR4, CCR5 or CD20 are available for overnight delivery to enable application optimization and small-scale testing.
Lipoparticles can be modified with (B) Biotin or (F) Fluorescence.
Featured Case Studies
Learn how Lipoparticles rescued a CX3CR1 discovery campaign and enabled a clinical stage antibody
Learn how Argenx used Lipoparticles to isolate antibodies with diverse epitopes against the GPCR glucagon receptor
Learn how AdAlta used our CXCR4 Lipoparticles to isolate i-bodies
Learn how Pfizer used Lipoparticles to target the CXCR4 pathway in leukemia
"Integral Molecular is the only source available for full length CXCR4 protein for ligand binding assays. Both human and mouse CXCR4 Lipoparticles are available, which was essential for the preclinical project that I was working on."
"The customer service is great and there are a wide variety of membrane proteins."
Frequently Asked Questions
Lipoparticles are noninfectious virus-like particles (VLPs) containing high concentrations of specific membrane proteins in their native conformation.
A Lipoparticle Unit correlates with the number of Lipoparticles in solution. One Unit is enough to obtain a strong ELISA response from 1 well of a 96-well plate. Lipoparticles are normalized by Dynamic Light Scattering (DLS). The DLS intensity is proportional to the number of Lipoparticles in solution.
Yes. Target membrane proteins are conformationally intact because they are incorporated onto the Lipoparticle surface directly from the cell surface and are never extracted, detergent solubilized, or refolded. Lipoparticles are usually produced from human cell lines, so receptors also contain native post-translational modifications. Conformational integrity is confirmed by binding to conformation-dependent antibodies or ligands, depending on the receptor and available reagents.
Yes, two or more membrane proteins can be incorporated simultaneously while maintaining their association as multiprotein complexes.
Lipoparticle stability is comparable to the stability of other purified proteins. Lipoparticles are stable for at least 2 years when refrigerated or frozen (-80°C). Like most lipid structures, Lipoparticles will be disrupted by detergents. Lipoparticle suspensions will appear as an opaque solution and should be gently mixed before use. Excessive vortexing, dilution, and filtration of Lipoparticles should be avoided to minimize sample loss.
Lipoparticles are nonliving biochemical reagents and can readily be modified during the production and purification process. Lipoparticles are available from Integral Molecular as biotinylated or fluorescently labeled molecules.
Lipoparticles bud from the cell surface, so they contain specific membrane proteins of interest as well as additional membrane proteins from the cells that are used for production. The effects of these nonspecific membrane proteins are minimized by the overexpression of the membrane protein of interest, the use of alternative cell types where necessary, and the use of Null Lipoparticles that do not contain the membrane protein of interest as negative controls.
Antibody Strategies for Membrane Protein Targets. 2009, Drug Discovery & Development
