NEUTRALIZATION ASSAY SERVICES

Virus neutralization data for your antibodies, including NT50

Critical virology reagents

20+ years virology expertise

Trusted by 100+ labs

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Do You Need Virus Neutralization Data for Your Antibodies?

Let us do the hard part. We have the facilities, the reagents, and the know-how to answer the most pressing questions about your antibodies. Work with us to save your team’s time, and get the data you need in as little as 4 weeks.

Antibodies and pseudovirus are mixed together in wells of a sample plate. Neutralizing antibodies bind to viral surface proteins and block infectivity.

To learn how to get started with a neutralization assay in your own lab, visit Pseudovirus Neutralization Assays: A how-to guide for getting started.

Test Against Any Variant in Our Catalog, and Then Some

Our neutralization assays are functional, cell-based assessments using non-replicative Reporter Virus Particles. A cell-based assay offers insight into the characteristics of an antibody that impact its clinical usefulness.

We have the tools to immediately assess your antibodies’ neutralizing properties against a wide variety of strains. Our Reporter Virus catalog is extensive. And we’re continually updating it with the latest SARS-CoV-2 variants of interest, seasonal influenza strains, and additional viruses with high pandemic potential.

If you need data for a variant not in our catalog, we can make it for you—and then run it in a neutralization assay.

Schematics of select reporter viruses from our catalog: Coronaviruses, including SARS-CoV-2 variants, SARS-CoV-1, and MERS-CoV-1; Influenza A and B, including seasonal and pandemic strains; Flaviviruses, including Zika, Dengue, and Yellow Fever; and Custom particles.

Reporter Virus Particles (RVPs) from Integral Molecular’s product offerings. Pseudovirus RVPs express viral surface proteins in a membrane surrounding a heterologous lentivirus core. They can express one surface protein (e.g., SARS-CoV-2) or multiple (e.g., influenza). Pseudoinfective particles (e.g., Dengue RVP) have surface and structural proteins from a virus of interest, but they lack genes essential for replication. All RVPs contain a GFP or luciferase reporter.

Popular Applications for Neutralization Assay Services

We do two types of neutralization assay projects. Neutralization Curve projects are for customers who want comparative neutralization data including NT50. Hits projects are a lower-cost option for customers who want to screen a panel of candidates for neutralizing activity.

Applications include the following:

  • Hit screening — test a panel to identify candidates with neutralizing activity
  • Antibody profiling — characterize candidates for hit validation or lead selection; test for neutralization against a panel of viral strains
  • Breadth of neutralization — test an antibody’s neutralizing ability against multiple viral strains
  • Antibody cocktail screening — test neutralization ability for combinations of antibodies
  • Validating predictions — generate data for molecules predicted by AI or modeling that may have virus-neutralizing activity
  • Small molecule screening — test for viral entry inhibition
Applications include the following:
Application
Description
Hit screening
Test a panel to identify candidates with neutralizing activity
Antibody profiling
Characterize candidates for hit validation or lead selection; test for neutralization against a panel of viral strains
Breadth of neutralization
Test an antibody’s neutralizing ability against multiple viral strains
Antibody cocktail screening
Test neutralization ability for combinations of antibodies
Validating predictions
Generate data for molecules predicted by AI or modeling that may have virus-neutralizing activity
Small molecule screening
Test for viral entry inhibition

Case Study: SARS-CoV-2 Reporter Viruses Reveal Antibodies’ Breadth of Neutralization

Two SARS-CoV-2 MAbs were tested for their ability to neutralize Reporter Virus Particles representing six viral variants. Each MAb showed a distinct neutralization profile.

Project Workflow

Before beginning a project, our experts work with you to understand your needs and develop a customized statement of work. Once the statement of work is approved, projects generally follow the workflow below.

As part of our commitment to quality, transparency, and timeliness, our team is available throughout the project to answer your questions. Should circumstances arise that could impact the project timeline, we will reach out with updates.

Once we receive antibodies or molecules, projects progress through three stages—experiment design and setup, neutralization assay completion, and data analysis—along with quality control checkpoints. Project deliverables include a report detailing neutralization or hits, and any data files used to generate the figures in your report.

Finding Answers to Your Antibody Questions

Just because an antibody can bind doesn’t mean it can neutralize. Our high-throughput screening platform can quickly and affordably identify which antibodies in your panel have neutralizing activity. When you leave the tedious part to us, your team has more time for critical activities.

Viruses are constantly evolving. When numerous variants exist, it’s important to understand how your antibody neutralizes all of them. As new variants emerge, updated neutralization data is critical to ensure ongoing potency for therapeutic antibodies and validity for other countermeasures. With our extensive catalog of off-the-shelf reagents, you can have data as soon as 4 weeks from receipt of antibodies. If you need to understand how your antibody neutralizes a special mutant or strain not in our catalog, we can prepare custom strains in as little as 10-12 weeks.

Neutralization curves and NT50 calculations are a snap for our team. We have years of experience with neutralization assays, and we routinely calculate NT50 on a test antibody each time we make a new lot of RVPs. To ensure the validity of your data, we will always test your antibodies alongside appropriate controls. You can trust us to get it right.

Featured Case Study

immunome logo

Learn how Immunome used SARS-CoV-2 variant RVPs to test their antibody cocktail

Immunome was developing a three-antibody cocktail with broad neutralization potency and a goal of retaining efficacy as the virus evolves. RVPs enabled the team to test their antibody cocktail against a large panel of viral variants of concern.

READ THE CASE STUDY

Contact Us for More Information

Webinar Recording: Innovative Research Tools to Combat Biological Threats

Topics include:

  • The use of pseudovirus Reporter Virus Particles (RVPs) in place of live viruses
  • How to drive the safe study of new virus variants and mutations of interest
  • Why innovative research tools play a critical role in the development of vaccines and treatments for a range of biological threats
  • Real-world case studies in discovering and characterizing neutralizing antibodies
WATCH THE VIDEO

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