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SOLUTIONS FOR VIROLOGY AND VACCINE DISCOVERY
Enabling the discovery of vaccines and antiviral therapeutics against SARS-CoV-2, dengue, Zika, and other viruses
The most extensive catalog of Reporter Virus Particles (RVPs)
Reporter Virus Particles (RVPs) are replication-incompetent pseudovirus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation
SARS-CoV-2 and Emergent Variant RVPs
SARS-CoV-2 / Variant | Catalog No. (G/L*) | Mutations | D614G |
|---|---|---|---|
Wuhan-Hu-1 | RVP-701 | Reference seq (GenBank QHD43416.1) | No |
D614G B.1, 20A | RVP-702 | D614G | Yes |
UK variant B.1.1.7, 20I/501Y.V1 | RVP-706 | ΔH69/V70, ΔY144, N501Y, A570D, P681H, T716I, S982A, D1118H | Yes |
UK variant B.1.1.7 with E484K | RVP-717 | ΔH69/V70, ΔY144, E484K, N501Y, A570D, P681H, T716I, S982A, D1118H | Yes |
South African variant Δ3
B.1.351, 20H/501Y.V2
| RVP-724 | L18F, D80A, D215G, ΔL242/A243/L244, R246I, K417N, N501Y, E484K, A701V | Yes |
South African variant B.1.351, RBD mutations only | RVP-715 | K417N, E484K, N501Y | Yes |
New York variant
B.1.526, 20C/484K
| RVP-726 | L5F, T95I, D253G, E484K, A701V | Yes |
Brazilian variant
P.1, 20J/501Y.V3
| RVP-708 | L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, T1027I, V1176F | Yes |
Nigerian/European variant
B.1.525, 20A/439K
| RVP-723 | Q52R, ΔH69/V70, ΔY144, E484K, Q677H, F888L | Yes |
Californian variant
B.1.427/B.1.429, 20C/452R
| RVP-713 | S13I, W152C, L452R | Yes |
*All RVPs are available with a GFP (G) or luciferase (L) reporter. Please add G/L to the catalog number when ordering to indicate the preferred reporter gene.
RVPs Coming Soon
SARS-CoV-2 Variant | Catalog No. (G/L) | Mutations | D614G |
|---|---|---|---|
UK/Nigerian variant B.1.1.318, 20B/484K | RVP-728 | T95I, ΔY144, E484K, P681H, D796H | Yes |
Indian variant B.1.617, RBD mutations only (“double mutant”) | RVP-727 | L452R, E484Q | Yes |
Indian variant B.1.617 | RVP-730 | G142D, E154K, L452R, E484Q, P681R, Q1071H | Yes |
Custom SARS-CoV-2 RVP variants and RVPs from our library of 1,200 alanine spike mutants are available upon request. Please contact us for pricing.
More SARS Variant RVPs
SARS-CoV-2 RVPs / Variants | Catalog No. (G/L) | Mutations | D614G |
|---|---|---|---|
US/Ohio strain, N501Y
20G/501Y
| RVP-710 | N501Y | Yes |
S477N (Australian/European variant)** | RVP-711 | S477N | Yes |
COHD15, US/Ohio variant | RVP-712 | Q677H, L938F | Yes |
Robin* 20G/677H | RVP-718 | Q677H | Yes |
Pelican* 20G/677P | RVP-719 | Q677P | Yes |
Quail* 20B/677H | RVP-721 | Q677H, T732S | Yes |
Mockingbird* 20A/677H | RVP-722 | G142S, E180V, Q677H | Yes |
E484K | RVP-709 | E484K | Yes |
N439K | RVP-703 | N439K | No |
N439K/D614G
20A/439K
| RVP-704 | N439K | Yes |
Danish mink “cluster 5” | RVP-705 | ΔH69/V70, Y453F, I692V, M1229I | No |
US variant N679S*** | RVP-725 | N679S | Yes |
South African variant
B.1.351, 20C/501Y.V2
| RVP-707 | L18F, D80A, D215G, L242H, R246I, K417N, N501Y, E484K, A701V | Yes |
South African variant Δ2
B.1.351, 20H/501Y.V2
| RVP-714 | L18F, D80A, D215G, ΔL242/A243, R246I, K417N, N501Y, E484K, A701V | Yes |
SARS-CoV-1 Urbani | RVP-801 | N/A | N/A |
MERS-CoV HCoV-EMC (GFP only) | RVP-901 | N/A | N/A |
* Described in Hodcroft et al., 2021
** Described in Hodcroft et al., 2020
***Identified in a newborn with high viral load (LoTempio et al., 2021)
See RVP application notes
Negative Control RVPs
Virus | Strain | Catalog No. (G/L) |
|---|---|---|
VSV | Indiana | RVP-1001 |
VSV RVPs can be used as a neutralization control to assess non-specific effects on infectivity or signal. These RVPs display the vesicular stomatitis virus glycoprotein, resulting in broad tropism, and show a single round of infectivity with numerous cell types, including the transgenic ACE2 cells provided with RVPs.
Cell Lines
Transgenes | Expression | Clonality | Origin Cell Line | Catalog No. |
|---|---|---|---|---|
hsACE2 | Stable | Monoclonal | HEK 293T | C-HA102 |
hsACE2, hsTMPRSS2 | Stable | Monoclonal | HEK 293T | C-HA201 |
Flavivirus RVPS
Virus | Strain | Catalog No. (G/L) |
|---|---|---|
Dengue Virus 1
| WestPac
| RVP-101
|
Dengue Virus 2
| S16803
| RVP-201
|
Dengue Virus 3
| CH53489
| RVP-301 |
Dengue Virus 4
| TVP360
| RVP-401
|
Zika Virus | SPH2015 | RVP-601 |
Integral Molecular’s RVPs are produced under stringent quality-controlled conditions
With two decades of virology experience, Integral Molecular is the industry leader in providing RVPs for applications including antibody R&D and serum screening for vaccine clinical trials
Frequently Asked Questions
Integral Molecular offers many vaccine development solutions including the production of reporter viruses, epitope mapping on viral targets, protein engineering for vaccine discovery and antibody discovery against viral targets.
RVPs incorporate virus-specific prM/E proteins and are antigenically equivalent to live wildtype viruses. They contain a modified RNA genome so they are safe and replication incompetent.
RVPs are useful for high-throughput infectivity and neutralization assays. They are an efficient alternative to plaque assays.
Luciferase or GFP is used for quantitative read out.
