Lipoparticles are provided with detailed protocols, application notes, and expert technical support to ensure your experiments succeed. Applications for Lipoparticles include:

• Immunization
• Phage and yeast display
• Screening and characterizing MAbs
• Biosensor binding kinetics
• Ligand binding assays
• Probing cells and microarrays

	The Lipoparticle Our quality control measures ensure that Lipoparticles and incorporated receptors remain intact for use in your experiments. Integral Molecular scientists continue to derive and test novel uses for Lipoparticles and will work with you to support new applications. Application Note: The Lipoparticle Application Note: Fluorescent and Biotin Modified Lipoparticles Application Note: Comparison to Membrane Preps Application Note: Lipoparticle Purity and Stability
	Antibody Development and Phage Panning Lipoparticles express conformationally-intact membrane proteins at concentrations 10-100 fold greater than conventional sources. Because Lipoparticle preparations do not contain impurities such as inverted membrane proteins or cytoplasmic proteins, they can elicit high titer antibodies against conformationally-complex membrane proteins. Biochemically modified Lipoparticles containing biotin tags or fluorophores enable membrane proteins to be captured on solid supports and visualized for use in flow cytometry based screening and phage display. Application Note: Lipoparticles as Immunogens
	Membrane Protein Interaction Kinetics: Biosensors The Lipoparticle's small size, high membrane protein concentration, and purity enable its use with optical biosensors such as ProteOn and Biacore biosensors. Association rate, dissociation rate, and affinity can be obtained, and panels of antibodies can be rapidly screened. Application Note: Biosensor Kinetic Analysis Application Note: Rapid Biosensor Screening Application Note: Array-based Biosensor Analysis Application Note: Lipoparticle Attachment to Biosensor Chips Application Note: One Shot Kinetic Analysis of Membrane Proteins Application Note: Kinetic Analysis of Mutated Proteins
	High Throughput Screening: Ligand Binding Lipoparticles contain structurally intact membrane proteins at high concentrations, offering improvements in assay sensitivity, specificity, and reproducibility compared with traditional formats such as whole cells or membrane preparations. Application Note: Radioligand Binding
	Probes and Sensors Lipoparticles are being used as membrane-delineated molecular probes, incorporating user-defined membrane-anchored proteins, soluble molecules, or enzymes for target interaction and optical signal emission. Application Note: The LipoProbe Application Note: Bead Binding

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Shotgun Mutagenesis Mapping

Shotgun Mutagenesis mapping enables detailed structure-function analysis of complex proteins, including epitope mapping and protein interactions with antibodies, drugs, and natural ligands.
Application Note: Shotgun Mutagenesis

	Antibody Epitope Mapping Identifying the structures that contribute to MAb binding sites (epitopes) can be rapidly achieved using Shotgun Mutagenesis. Epitope maps are commonly used in selecting the most relevant MAbs for therapeutic, diagnostic (e.g. biomarker), and research applications, including vaccine development. Application Note: Epitope Mapping Application Note: Atomic-level Epitope Mapping Application Note: Epitope Intellectual Property Protection
	Mapping Drug Binding Sites Shotgun Mutagenesis rapidly generates maps of the critical residues required for drug binding. This SAR information can be used to guide computational docking studies to design improved small molecules, and to differentiate intellectual property positions of otherwise similar compounds. Application Note: Drug Mapping and Docking
	Identifying Functional and Signaling Regions Because Shotgun Mutagenesis enables the function of protein mutations to be tested in living eukaryotic cells, functional domains within a protein can be explored using standard microplate-based assays. Residues required for specific functions, such as GPCR signaling and virus receptor usage, are readily identified in this way. Application Note: VIrus Receptor Mapping Application Note: Ligand Mapping

 

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Reporter Virus Particles (RVPs)

RVPs are replication-incompetent virus particles that enable viral infectivity studies to be conducted safely, easily, and reproducibly using standard detection instrumentation. RVPs express convenient optical reporter genes upon cellular infection and are offered as quality-controlled, ready-to-use reagents.

Dengue RVPs Dengue RVPs retain antigenic equivalence to wild-type virions by incorporating specific prME proteins from Dengue serotypes 1, 2, 3, or 4. Dengue RVPs feature a luminescent reporter that provides a rapid and convenient read-out of infectivity, and are validated for both antibody neutralization and enhancement experiments. Application Note: Dengue RVP Neutralization Assay Application Note: Dengue RVP Enhancement Assay Application Note: Dengue RVP Stability Application Note: Dengue RVP Reproducibility